› 2qhr

X-ray diffraction
2Å resolution

Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.

Released:
DOI: 10.2210/pdb2qhr/pdb
PDB entry 2qhr coloured by chain, front view.
PDB entry 2qhr coloured by chain, side view.
PDB entry 2qhr coloured by chain, top view.
Source organisms:
Primary publication:
Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain.
Lee JE, Kuehne A, Abelson DM, Fusco ML, Hart MK, Saphire EO
J Mol Biol 375 202-16 (2008)
PMID: 18005986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208570 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
13F6-1-2 Fab fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.99 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig lambda-2 chain C region Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.63 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01844 (Residues: 1-102; Coverage: 98%)
Gene name: Iglc2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
GP1 Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.41 KDa
Source organism: Ebola virus - Eckron (Zaire, 1976)
Expression system: Not provided
UniProt:
  • Canonical: P87671 (Residues: 404-414; Coverage: 2%)
Gene name: GP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 68.24Å b: 70.3Å c: 142.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.245
Expression system: Not provided

Quick links

Citations

8 review citations

Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
Wlodawer et al. (2008)
7 more

13 mentions without citation

Neutralizing ebolavirus: structural insights into the envelope glycoprotein and antibodies targeted against it.
Lee et al. (2009)
12 more