› 2qjp

X-ray diffraction
2.6Å resolution

Crystal structure of wild type rhodobacter sphaeroides with stigmatellin and antimycin inhibited

Released:
DOI: 10.2210/pdb2qjp/pdb
PDB entry 2qjp coloured by chain, front view.
PDB entry 2qjp coloured by chain, side view.
PDB entry 2qjp coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Inhibitor-complexed structures of the cytochrome bc1 from the photosynthetic bacterium Rhodobacter sphaeroides.
Esser L, Elberry M, Zhou F, Yu CA, Yu L, Xia D
J Biol Chem 283 2846-57 (2008)
PMID: 18039651

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-169747 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome b Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 428 amino acids
Theoretical weight: 48.37 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02761 (Residues: 3-430; Coverage: 96%)
Gene names: fbcB, petB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1 Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 256 amino acids
Theoretical weight: 27.79 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02760 (Residues: 23-278; Coverage: 97%)
Gene names: fbcC, petC
Sequence domains: Cytochrome C1 family
Structure domains:
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 179 amino acids
Theoretical weight: 19.07 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02762 (Residues: 9-187; Coverage: 96%)
Gene names: fbcF, petA
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand BGL 4 x BGL
Ligand HEM 12 x HEM
Ligand SMA 4 x SMA
Ligand LOP 4 x LOP
Ligand ANJ 4 x ANJ
Ligand SR 6 x SR
Ligand FES 4 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 135.061Å b: 146.52Å c: 141.003Å
α: 90° β: 110.21° γ: 90°
R-values:
R R work R free
0.244 0.244 0.277
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Superoxide generation by complex III: from mechanistic rationales to functional consequences.
Bleier et al. (2013)
18 more

17 mentions without citation

Structural analysis of cytochrome bc1 complexes: implications to the mechanism of function.
Xia et al. (2013)
16 more