› 2qlw

X-ray diffraction
1.6Å resolution

Crystal structure of rhamnose mutarotase RhaU of Rhizobium leguminosarum

Released:
DOI: 10.2210/pdb2qlw/pdb
PDB entry 2qlw coloured by chain, front view.
PDB entry 2qlw coloured by chain, side view.
PDB entry 2qlw coloured by chain, top view.
Source organism: Rhizobium leguminosarum bv. trifolii
Primary publication:
RhaU of Rhizobium leguminosarum is a rhamnose mutarotase.
Richardson JS, Carpena X, Switala J, Perez-Luque R, Donald LJ, Loewen PC, Oresnik IJ
J Bacteriol 190 2903-10 (2008)
PMID: 18156270

Function and Biology Details

Reaction catalysed: 
Alpha-L-rhamnopyranose = beta-L-rhamnopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-181372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-rhamnose mutarotase Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 17 KDa
Source organism: Rhizobium leguminosarum bv. trifolii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BSH1 (Residues: 2-106; Coverage: 99%)
Gene names: rhaM, rhaU
Sequence domains: L-rhamnose mutarotase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand FMT 16 x FMT
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P3212
Unit cell:
a: 69.241Å b: 69.241Å c: 101.106Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.152 0.18
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Physiology, genetics, and biochemistry of carbon metabolism in the alphaproteobacterium Sinorhizobium meliloti.
Geddes et al. (2014)
11 other articles

1 mention without citation

RhaU of Rhizobium leguminosarum is a rhamnose mutarotase.
Richardson et al. (2008)