› 2qm3

X-ray diffraction
2.05Å resolution

Crystal structure of a predicted methyltransferase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb2qm3/pdb
PDB entry 2qm3 coloured by chain, front view.
PDB entry 2qm3 coloured by chain, side view.
PDB entry 2qm3 coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Entry authors: Cuff ME, Duggan E, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = S-methyl-5'-thioadenosine + N(4)-aminopropylspermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N(4)-bis(aminopropyl)spermidine synthase Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 43.35 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U1U4 (Residues: 1-349; Coverage: 100%)
Gene names: PF1111, bpsA
Sequence domains: Branched-chain polyamine synthase A C-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ACY 3 x ACY
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 82.382Å b: 85.262Å c: 53.963Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.227
Expression system: Escherichia coli BL21(DE3)

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