› 2qmt

X-ray diffraction
1.05Å resolution

Crystal Polymorphism of Protein GB1 Examined by Solid-state NMR and X-ray Diffraction

Released:
DOI: 10.2210/pdb2qmt/pdb
PDB entry 2qmt coloured by chain, front view.
PDB entry 2qmt coloured by chain, side view.
PDB entry 2qmt coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal polymorphism of protein GB1 examined by solid-state NMR spectroscopy and X-ray diffraction.
Schmidt HL, Sperling LJ, Gao YG, Wylie BJ, Boettcher JM, Wilson SR, Rienstra CM
J Phys Chem B 111 14362-9 (2007)
PMID: 18052145

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.23 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand MRD 1 x MRD
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3221
Unit cell:
a: 35.73Å b: 35.73Å c: 75.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.182 0.207
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Polymorphism and solvatomorphism 2007.
Brittain HG. (2009)
1 more

57 mentions without citation

Systematic Targeting of Protein-Protein Interactions.
Modell et al. (2016)
56 more