› 2qne

X-ray diffraction
2.3Å resolution

Crystal structure of putative methyltransferase (ZP_00558420.1) from Desulfitobacterium hafniense Y51 at 2.30 A resolution

Released:
DOI: 10.2210/pdb2qne/pdb
PDB entry 2qne coloured by chain, front view.
PDB entry 2qne coloured by chain, side view.
PDB entry 2qne coloured by chain, top view.
Source organism: Desulfitobacterium hafniense Y51
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Glycine betaine + a [Co(I) glycine betaine-specific corrinoid protein] = N,N-dimethylglycine + a [methyl-Co(III) glycine betaine-specific corrinoid protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine betaine methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 495 amino acids
Theoretical weight: 55.57 KDa
Source organism: Desulfitobacterium hafniense Y51
Expression system: Escherichia coli
UniProt:
  • Canonical: Q24SP7 (Residues: 4-479; Coverage: 99%)
Gene names: DSY3156, mtgB
Sequence domains: Trimethylamine methyltransferase (MTTB)
Structure domains: Trimethylamine methyltransferase-like

Ligands and Environments

1 bound ligand:
Ligand EDO 10 x EDO
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3221
Unit cell:
a: 123.863Å b: 123.863Å c: 122.844Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.158 0.204
Expression system: Escherichia coli

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