› 2qo4

X-ray diffraction
1.5Å resolution

Crystal structure of zebrafish liver bile acid-binding protein complexed with cholic acid

Released:
DOI: 10.2210/pdb2qo4/pdb
PDB entry 2qo4 coloured by chain, front view.
PDB entry 2qo4 coloured by chain, side view.
PDB entry 2qo4 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
A single amino acid mutation in zebrafish (Danio rerio) liver bile acid-binding protein can change the stoichiometry of ligand binding.
Capaldi S, Guariento M, Saccomani G, Fessas D, Perduca M, Monaco HL
J Biol Chem 282 31008-18 (2007)
PMID: 17670743

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid-binding protein 10-A, liver basic Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.02 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I8L5 (Residues: 1-126; Coverage: 100%)
Gene names: fabp10, fabp10a, zgc:103719, zgc:92741
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

3 bound ligands:
Ligand CHD 1 x CHD
Ligand IPA 3 x IPA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P32
Unit cell:
a: 43.355Å b: 43.355Å c: 67.526Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.209
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A survey of the year 2007 literature on applications of isothermal titration calorimetry.
Bjelić et al. (2008)
3 more

3 mentions without citation

Rosetta:MSF: a modular framework for multi-state computational protein design.
Löffler et al. (2017)
2 more