› 2qoj

X-ray diffraction
2.4Å resolution

Coevolution of a homing endonuclease and its host target sequence

Released:
DOI: 10.2210/pdb2qoj/pdb
PDB entry 2qoj coloured by chain, front view.
PDB entry 2qoj coloured by chain, side view.
PDB entry 2qoj coloured by chain, top view.
Source organism: Aspergillus nidulans FGSC A4
Primary publication:
Coevolution of a homing endonuclease and its host target sequence.
Scalley-Kim M, McConnell-Smith A, Stoddard BL
J Mol Biol 372 1305-19 (2007)
PMID: 17720189

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115810 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease/RNA maturase I-AniI Chain: Z
Molecule details ›
Chain: Z
Length: 254 amino acids
Theoretical weight: 29.62 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Escherichia coli
UniProt:
  • Canonical: P03880 (Residues: 235-488; Coverage: 52%)
Gene name: I-AniI
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
I-AniI DNA target seq1 Chain: X
Molecule details ›
Chain: X
Length: 31 nucleotides
Theoretical weight: 9.61 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Not provided
I-AniI DNA target seq2 Chain: Y
Molecule details ›
Chain: Y
Length: 31 nucleotides
Theoretical weight: 9.46 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 60.353Å b: 72.701Å c: 61.1Å
α: 90° β: 103.88° γ: 90°
R-values:
R R work R free
0.219 0.216 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity.
Chan et al. (2011)
9 more

9 mentions without citation

Exploitation of binding energy for catalysis and design.
Thyme et al. (2009)
8 more