› 2qpa

X-ray diffraction
3.2Å resolution

Crystal Structure of S.cerevisiae Vps4 in the presence of ADP

Released:
DOI: 10.2210/pdb2qpa/pdb
PDB entry 2qpa coloured by chain, front view.
PDB entry 2qpa coloured by chain, side view.
PDB entry 2qpa coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural characterization of the ATPase reaction cycle of endosomal AAA protein Vps4.
Xiao J, Xia H, Yoshino-Koh K, Zhou J, Xu Z
J Mol Biol 374 655-70 (2007)
PMID: 17949747

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 355 amino acids
Theoretical weight: 38.78 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P52917 (Residues: 83-437; Coverage: 81%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 75.63Å b: 120.64Å c: 157.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.305
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The ESCRT complexes.
Hurley JH. (2010)
7 more

6 mentions without citation

Biochemical and structural studies of yeast Vps4 oligomerization.
Gonciarz et al. (2008)
5 more