› 2qpl

X-ray diffraction
2.1Å resolution

Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue

Released:
DOI: 10.2210/pdb2qpl/pdb
PDB entry 2qpl coloured by chain, front view.
PDB entry 2qpl coloured by chain, side view.
PDB entry 2qpl coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue.
Pereira HM, Berdini V, Cleasby A, Garratt RC
FEBS Lett 581 5082-6 (2007)
PMID: 17927987

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 31.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P55859 (Residues: 3-284; Coverage: 98%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand MG 1 x MG
Ligand BTY 1 x BTY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P213
Unit cell:
a: 93.442Å b: 93.442Å c: 93.442Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.218

Quick links

Citations

2 review citations

Docking and chemoinformatic screens for new ligands and targets.
Kolb et al. (2009)
1 more

1 mention without citation

Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination.
Pécsi et al. (2011)