› 2qrw

X-ray diffraction
1.93Å resolution

Crystal structure of Mycobacterium tuberculosis trHbO WG8F mutant

Released:
DOI: 10.2210/pdb2qrw/pdb
PDB entry 2qrw coloured by chain, front view.
PDB entry 2qrw coloured by chain, side view.
PDB entry 2qrw coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The roles of Tyr(CD1) and Trp(G8) in Mycobacterium tuberculosis truncated hemoglobin O in ligand binding and on the heme distal site architecture.
Ouellet H, Milani M, LaBarre M, Bolognesi M, Couture M, Guertin M
Biochemistry 46 11440-50 (2007)
PMID: 17887774

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-161824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Group 2 truncated hemoglobin GlbO Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 128 amino acids
Theoretical weight: 14.93 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WN23 (Residues: 1-128; Coverage: 100%)
Gene names: MTV008.26, Rv2470, glbO
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand CYN 12 x CYN
Ligand SO4 23 x SO4
Ligand HEM 12 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: I4122
Unit cell:
a: 187.312Å b: 187.312Å c: 274.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Escherichia coli

Quick links

Citations

19 citation in other articles

Ligand migration in the truncated hemoglobin-II from Mycobacterium tuberculosis: the role of G8 tryptophan.
Guallar et al. (2009)
18 more

1 mention without citation

Transplanting supersites of HIV-1 vulnerability.
Zhou et al. (2014)