› 2qsi

X-ray diffraction
1.8Å resolution

Crystal structure of putative hydrogenase expression/formation protein hupG from Rhodopseudomonas palustris CGA009

Released:
DOI: 10.2210/pdb2qsi/pdb
PDB entry 2qsi coloured by chain, front view.
PDB entry 2qsi coloured by chain, side view.
PDB entry 2qsi coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Entry authors: Nocek B, Skarina T, Kagan O, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179999 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrogenase expression/formation protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 14.46 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6NB62 (Residues: 1-133; Coverage: 100%)
Gene names: RPA0968, TX73_004985, hupG
Sequence domains: Hydrogenase-1 expression protein HyaE
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DTT 2 x DTT
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 31.575Å b: 41.126Å c: 46.85Å
α: 107.72° β: 99.85° γ: 101.75°
R-values:
R R work R free
0.156 0.152 0.201
Expression system: Escherichia coli BL21(DE3)

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