› 2qt6

X-ray diffraction
1.5Å resolution

Crystal Structure Determination of a Blue Laccase from Lentinus Tigrinus

Released:
DOI: 10.2210/pdb2qt6/pdb
PDB entry 2qt6 coloured by chain, front view.
PDB entry 2qt6 coloured by chain, side view.
PDB entry 2qt6 coloured by chain, top view.
Source organism: Lentinus tigrinus
Primary publication:
Crystal structure of a blue laccase from Lentinus tigrinus: evidences for intermediates in the molecular oxygen reductive splitting by multicopper oxidases.
Ferraroni M, Myasoedova NM, Schmatchenko V, Leontievsky AA, Golovleva LA, Scozzafava A, Briganti F
BMC Struct Biol 7 60 (2007)
PMID: 17897461

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Laccase Chains: A, B
Molecule details ›
Chains: A, B
Length: 498 amino acids
Theoretical weight: 53.98 KDa
Source organism: Lentinus tigrinus
UniProt:
  • Canonical: Q5EBY5 (Residues: 1-463; Coverage: 100%)
Gene name: lcc1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
8 bound ligands:
Ligand CU 8 x CU
Ligand NAG 1 x NAG
Ligand TLA 1 x TLA
Ligand GOL 2 x GOL
Ligand MAN 2 x MAN
Ligand CA 6 x CA
Ligand CL 3 x CL
Ligand PER 1 x PER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21
Unit cell:
a: 54.218Å b: 111.613Å c: 97.086Å
α: 90° β: 97.75° γ: 90°
R-values:
R R work R free
0.15 0.15 0.182

Quick links

Citations

11 review citations

Laccases: a never-ending story.
Giardina et al. (2010)
10 more

11 mentions without citation

Crystal structure of a blue laccase from Lentinus tigrinus: evidences for intermediates in the molecular oxygen reductive splitting by multicopper oxidases.
Ferraroni et al. (2007)
10 more