› 2qtd

X-ray diffraction
1.7Å resolution

Crystal structure of a putative dinitrogenase (mj0327) from methanocaldococcus jannaschii dsm at 1.70 A resolution

Released:
DOI: 10.2210/pdb2qtd/pdb
PDB entry 2qtd coloured by chain, front view.
PDB entry 2qtd coloured by chain, side view.
PDB entry 2qtd coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
NMR structure of the protein NP_247299.1: comparison with the crystal structure.
Jaudzems K, Geralt M, Serrano P, Mohanty B, Horst R, Pedrini B, Elsliger MA, Wilson IA, Wüthrich K
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 1367-80 (2010)
PMID: 20944234

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein MJ0327 Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 12.11 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57773 (Residues: 1-104; Coverage: 100%)
Gene name: MJ0327
Sequence domains: Dinitrogenase iron-molybdenum cofactor
Structure domains: Dinitrogenase iron-molybdenum cofactor biosynthesis domain

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand PG4 7 x PG4
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 35.86Å b: 54.12Å c: 56.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.255
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Hinge-shift mechanism as a protein design principle for the evolution of β-lactamases from substrate promiscuity to specificity.
Modi et al. (2021)
4 more

1 mention without citation

NMR structure of the protein NP_247299.1: comparison with the crystal structure.
Jaudzems et al. (2010)