› 2qu1

X-ray diffraction
1.7Å resolution

Crystal Structure of a Cyclized GFP Variant

Released:
DOI: 10.2210/pdb2qu1/pdb
PDB entry 2qu1 coloured by chain, front view.
PDB entry 2qu1 coloured by chain, side view.
PDB entry 2qu1 coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Bailey LJ, McCoy JG, Bitto E, Bingman CA, Fox BG, Wesenberg GE, Phillips Jr GN, Center for Eukaryotic Structural Genomics (CESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.25 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
2 modified residues:
Ligand MSE 5 x MSE
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 51.46Å b: 61.99Å c: 70.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.22
Expression system: Escherichia coli

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