2qu7

X-ray diffraction
2.3Å resolution

Crystal structure of a putative transcription regulator from Staphylococcus saprophyticus subsp. saprophyticus

Released:
Entry authors: Bonanno JB, Freeman J, Bain KT, Mendoza M, Romero R, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-175426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 32.35 KDa
Source organism: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4A0A0 (Residues: 60-336; Coverage: 82%)
Gene name: SSP0362
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21212
Unit cell:
a: 93.417Å b: 116.382Å c: 68.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.268
Expression system: Escherichia coli BL21(DE3)