› 2qub

X-ray diffraction
1.8Å resolution

Crystal structure of extracellular lipase LipA from Serratia marcescens

Released:
DOI: 10.2210/pdb2qub/pdb
PDB entry 2qub coloured by chain, front view.
PDB entry 2qub coloured by chain, side view.
PDB entry 2qub coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens.
Meier R, Drepper T, Svensson V, Jaeger KE, Baumann U
J Biol Chem 282 31477-83 (2007)
PMID: 17728256

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular lipase Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 615 amino acids
Theoretical weight: 65.07 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59933 (Residues: 1-613; Coverage: 100%)
Gene name: lipA
Sequence domains: RTX calcium-binding nonapeptide repeat (4 copies)
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand CA 48 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 202.401Å b: 202.401Å c: 317.731Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.171 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Advances in lipase-catalyzed esterification reactions.
Stergiou et al. (2013)
7 more

5 mentions without citation

Re-evaluation of a bacterial antifreeze protein as an adhesin with ice-binding activity.
Guo et al. (2012)
4 more