› 2qv8

X-ray diffraction
2Å resolution

Structure of the minor pseudopilin EpsH from the Type 2 Secretion System of Vibrio cholerae

Released:
DOI: 10.2210/pdb2qv8/pdb
PDB entry 2qv8 coloured by chain, front view.
PDB entry 2qv8 coloured by chain, side view.
PDB entry 2qv8 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae.
Yanez ME, Korotkov KV, Abendroth J, Hol WG
J Mol Biol 377 91-103 (2008)
PMID: 18241884

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein H Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.21 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45774 (Residues: 36-194; Coverage: 82%)
Gene names: VC_2729, epsH
Sequence domains: Type II transport protein GspH
Structure domains: minor pseudopilin epsh domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 53.349Å b: 70.449Å c: 85.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Type II secretion system: a magic beanstalk or a protein escalator.
Nivaskumar et al. (2014)
6 more

6 mentions without citation

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
5 more