Function and Biology Details
Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178866 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb protein EED
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Length: 361 amino acids
Theoretical weight: 41.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
Q921E6 (Residues: 81-441; Coverage: 82%)
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone-lysine N-methyltransferase EZH2
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: WD repeat binding protein EZH2
Length: 30 amino acids
Theoretical weight: 3.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
- Canonical: Q61188 (Residues: 39-68; Coverage: 4%)
Sequence domains: WD repeat binding protein EZH2
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
BSRF BEAMLINE 3W1A
Spacegroup:
P212121
Expression system: Escherichia coli BL21
