› 2qy6

X-ray diffraction
2Å resolution

Crystal structure of the N-terminal domain of UPF0209 protein yfcK from Escherichia coli O157:H7

Released:
DOI: 10.2210/pdb2qy6/pdb
PDB entry 2qy6 coloured by chain, front view.
PDB entry 2qy6 coloured by chain, side view.
PDB entry 2qy6 coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Entry authors: Bonanno JB, Dickey M, Bain KT, Eberle M, Ozyurt S, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + tRNA containing 5-(aminomethyl)-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing ((5-methylamino)methyl)-2-thiouridylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.47 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XCQ7 (Residues: 2-247; Coverage: 37%)
Gene names: ECs3208, Z3587, mnmC
Sequence domains: S-adenosyl-L-methionine-dependent methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 72.067Å b: 80.468Å c: 234.376Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links