› 2qyu

X-ray diffraction
2.1Å resolution

Crystal structure of Salmonella effector protein SopA

Released:
DOI: 10.2210/pdb2qyu/pdb
Model geometry
Fit model/data
PDB entry 2qyu coloured by chain, front view.
PDB entry 2qyu coloured by chain, side view.
PDB entry 2qyu coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Primary publication:
Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase.
Diao J, Zhang Y, Huibregtse JM, Zhou D, Chen J
Nat Struct Mol Biol 15 65-70 (2008)
PMID: 18066077

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase SopA Chain: A
Molecule details ›
Chain: A
Length: 627 amino acids
Theoretical weight: 70.1 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZNR3 (Residues: 163-782; Coverage: 79%)
Gene names: STM2066, sopA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand B3P 1 x B3P
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P4122
Unit cell:
a: 79.72Å b: 79.72Å c: 212.731Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Ubiquitin Ligases: Structure, Function, and Regulation.
Zheng et al. (2017)
38 more

11 mentions without citation

Bacteria-host relationship: ubiquitin ligases as weapons of invasion.
Maculins et al. (2016)
10 more