› 2r11

X-ray diffraction
1.96Å resolution

Crystal structure of putative hydrolase (2632844) from Bacillus subtilis at 1.96 A resolution

Released:
DOI: 10.2210/pdb2r11/pdb
PDB entry 2r11 coloured by chain, front view.
PDB entry 2r11 coloured by chain, side view.
PDB entry 2r11 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized carboxylesterase nap Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 306 amino acids
Theoretical weight: 35.32 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P96688 (Residues: 1-294; Coverage: 98%)
Gene names: BSU05440, nap
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand MG 1 x MG
Ligand EDO 2 x EDO
Ligand PGE 1 x PGE
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 80.59Å b: 97.55Å c: 88.27Å
α: 90° β: 110.01° γ: 90°
R-values:
R R work R free
0.184 0.181 0.239
Expression system: Escherichia coli

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