› 2r20

X-ray diffraction
1.3Å resolution

Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at 1.3 A (Br2)

Released:
DOI: 10.2210/pdb2r20/pdb
PDB entry 2r20 coloured by chain, front view.
PDB entry 2r20 coloured by chain, side view.
PDB entry 2r20 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
The 1.3 A resolution structure of the RNA tridecamer r(GCGUUUGAAACGC): metal ion binding correlates with base unstacking and groove contraction.
Timsit Y, Bombard S
RNA 13 2098-107 (2007)
PMID: 17940138

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196577 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*GP*CP*GP*UP*UP*(5BU)P*GP*AP*AP*AP*CP*GP*C)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 13 nucleotides
Theoretical weight: 4.24 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 5 x NA
Ligand MG 1 x MG
1 modified residue:
Ligand 5BU 2 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 25.28Å b: 25.8Å c: 29.99Å
α: 98.62° β: 105.58° γ: 100.92°
R-values:
R R work R free
0.181 0.181 0.195
Expression system: Not provided

Quick links

Citations

6 citation in other articles

Dependence of A-RNA simulations on the choice of the force field and salt strength.
Besseová et al. (2009)
5 more

5 mentions without citation

Refinement of the Cornell et al. Nucleic Acids Force Field Based on Reference Quantum Chemical Calculations of Glycosidic Torsion Profiles.
Zgarbová et al. (2011)
4 more