› 2r2i

X-ray diffraction
2Å resolution

Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound

Released:
DOI: 10.2210/pdb2r2i/pdb
PDB entry 2r2i coloured by chain, front view.
PDB entry 2r2i coloured by chain, side view.
PDB entry 2r2i coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1.
Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC
Structure 15 1392-402 (2007)
PMID: 17997965

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylyl cyclase-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.73 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P79880 (Residues: 2-199; Coverage: 100%)
Gene names: GCAP1, GUCA1A
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand MYR 1 x MYR
Ligand BME 4 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2, ALS BEAMLINE 8.3.1
Spacegroup: P41212
Unit cell:
a: 73.333Å b: 73.333Å c: 73.229Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.251
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Chemistry and biology of vision.
Palczewski K. (2012)
16 more

27 mentions without citation

Molecular structure and target recognition of neuronal calcium sensor proteins.
Ames et al. (2012)
26 more