› 2r2v

X-ray diffraction
1.9Å resolution

Sequence Determinants of the Topology of the Lac Repressor Tetrameric Coiled Coil

Released:
DOI: 10.2210/pdb2r2v/pdb
PDB entry 2r2v coloured by chain, front view.
PDB entry 2r2v coloured by chain, side view.
PDB entry 2r2v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Conformational specificity of the lac repressor coiled-coil tetramerization domain.
Liu J, Zheng Q, Deng Y, Li Q, Kallenbach NR, Lu M
Biochemistry 46 14951-9 (2007)
PMID: 18052214

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GCN4 leucine zipper Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 34 amino acids
Theoretical weight: 3.87 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ACT 3 x ACT
Ligand CIT 4 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43
Unit cell:
a: 51.696Å b: 51.696Å c: 99.793Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.24
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Structural specificity in coiled-coil interactions.
Grigoryan et al. (2008)
13 other articles

4 mentions without citation

The Structure and Topology of α-Helical Coiled Coils.
Lupas et al. (2017)
3 more