› 2r42

X-ray diffraction
2.4Å resolution

The Biochemical and Structural Basis for feedback Inhibition of Mevalonate Kinase and Isoprenoid Metabolism

Released:
DOI: 10.2210/pdb2r42/pdb
PDB entry 2r42 coloured by chain, front view.
PDB entry 2r42 coloured by chain, side view.
PDB entry 2r42 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Biochemical and structural basis for feedback inhibition of mevalonate kinase and isoprenoid metabolism.
Fu Z, Voynova NE, Herdendorf TJ, Miziorko HM, Kim JJ
Biochemistry 47 3715-24 (2008)
PMID: 18302342

Function and Biology Details

Reaction catalysed: 
ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mevalonate kinase Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 42.03 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17256 (Residues: 1-395; Coverage: 100%)
Gene name: Mvk
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand FPS 1 x FPS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 77.99Å b: 118.43Å c: 42.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
3 more