› 2r48

X-ray diffraction
1.8Å resolution

Crystal structure of the fructose specific IIB subunit of PTS system from Bacillus subtilis subsp. subtilis str. 168

Released:
DOI: 10.2210/pdb2r48/pdb
PDB entry 2r48 coloured by chain, front view.
PDB entry 2r48 coloured by chain, side view.
PDB entry 2r48 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Nocek B, Cuff M, Sather A, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-mannose(Side 1) = [protein]-L-histidine + D-mannose 6-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system mannose-specific EIIBCA component Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.48 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31645 (Residues: 2-104; Coverage: 16%)
Gene names: BSU12010, manP, yjdD
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 48.931Å b: 48.931Å c: 68.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.183 0.237
Expression system: Escherichia coli BL21(DE3)

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