› 2r4q

X-ray diffraction
1.6Å resolution

The structure of a domain of fruA from Bacillus subtilis

Released:
DOI: 10.2210/pdb2r4q/pdb
PDB entry 2r4q coloured by chain, front view.
PDB entry 2r4q coloured by chain, side view.
PDB entry 2r4q coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Cuff ME, Sather A, Nocek B, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system fructose-specific EIIABC component Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.61 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71012 (Residues: 171-273; Coverage: 16%)
Gene names: BSU14400, fruA
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P43212
Unit cell:
a: 43.661Å b: 43.661Å c: 110.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.233
Expression system: Escherichia coli BL21(DE3)

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