› 2r5m

X-ray diffraction
2.65Å resolution

Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with peptide R-(me)K-S

Released:
DOI: 10.2210/pdb2r5m/pdb
PDB entry 2r5m coloured by chain, front view.
PDB entry 2r5m coloured by chain, side view.
PDB entry 2r5m coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg.
Grimm C, de Ayala Alonso AG, Rybin V, Steuerwald U, Ly-Hartig N, Fischle W, Müller J, Müller CW
EMBO Rep 8 1031-7 (2007)
PMID: 17932512

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163216 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb protein Scm Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.4 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VHA0 (Residues: 175-435; Coverage: 30%)
Gene names: CG9495, Scm
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.
peptide R(me)KS Chain: L
Molecule details ›
Chain: L
Length: 3 amino acids
Theoretical weight: 404 Da
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLZ 1 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 82.71Å b: 82.71Å c: 76.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.169 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Histones: annotating chromatin.
Campos et al. (2009)
9 more

3 mentions without citation

Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.
McElroy et al. (2014)
2 more