› 2r5r

X-ray diffraction
3.05Å resolution

The crystal structure of DUF198 from Nitrosomonas europaea ATCC 19718

Released:
DOI: 10.2210/pdb2r5r/pdb
PDB entry 2r5r coloured by chain, front view.
PDB entry 2r5r coloured by chain, side view.
PDB entry 2r5r coloured by chain, top view.
Source organism: Nitrosomonas europaea ATCC 19718
Entry authors: Tan K, Wu R, Nocek B, Bigelow L, Patterson S, Freeman L, Bargassa M, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182705 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP cyclohydrolase FolE2 Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.2 KDa
Source organism: Nitrosomonas europaea ATCC 19718
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q82VD1 (Residues: 1-268; Coverage: 100%)
Gene names: NE1163, folE2
Sequence domains: Type I GTP cyclohydrolase folE2
Structure domains: Urate Oxidase;

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand IMD 1 x IMD
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6422
Unit cell:
a: 118.705Å b: 118.705Å c: 113.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.239 0.261
Expression system: Escherichia coli BL21

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