› 2r5u

X-ray diffraction
1.9Å resolution

Crystal structure of the N-terminal domain of DnaB helicase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2r5u/pdb
PDB entry 2r5u coloured by chain, front view.
PDB entry 2r5u coloured by chain, side view.
PDB entry 2r5u coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Hexameric ring structure of the N-terminal domain of Mycobacterium tuberculosis DnaB helicase.
Biswas T, Tsodikov OV
FEBS J 275 3064-71 (2008)
PMID: 18479467

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicative DNA helicase, 1st part Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 200 amino acids
Theoretical weight: 21.37 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMR3 (Residues: 1-197; Coverage: 23%)
Gene names: MTCY21D4.21, Rv0058, dnaB
Sequence domains: DnaB-like helicase N terminal domain
Structure domains: DNAb Helicase; Chain A

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P31
Unit cell:
a: 114.927Å b: 114.927Å c: 72.393Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
5 more

8 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
7 more