› 2r5y

X-ray diffraction
2.6Å resolution

Structure of Scr/Exd complex bound to a consensus Hox-Exd site

Released:
DOI: 10.2210/pdb2r5y/pdb
PDB entry 2r5y coloured by chain, front view.
PDB entry 2r5y coloured by chain, side view.
PDB entry 2r5y coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Functional specificity of a Hox protein mediated by the recognition of minor groove structure.
Joshi R, Passner JM, Rohs R, Jain R, Sosinsky A, Crickmore MA, Jacob V, Aggarwal AK, Honig B, Mann RS
Cell 131 530-43 (2007)
PMID: 17981120

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115820 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeotic protein Sex combs reduced Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.97 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P09077 (Residues: 298-384; Coverage: 21%)
Gene names: CG1030, Scr
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
Homeobox protein extradenticle Chain: B
Molecule details ›
Chain: B
Length: 63 amino acids
Theoretical weight: 7.55 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P40427 (Residues: 238-300; Coverage: 17%)
Gene names: CG8933, exd
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
DNA (5'-D(*DAP*DCP*DTP*DCP*DTP*DAP*DTP*DGP*DAP*DTP*DTP*DTP*DAP*DTP*DGP*DGP*DGP*DCP*DTP*DG)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
DNA (5'-D(*DTP*DCP*DAP*DGP*DCP*DCP*DCP*DAP*DTP*DAP*DAP*DAP*DTP*DCP*DAP*DTP*DAP*DGP*DAP*DG)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.11 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 65.31Å b: 65.31Å c: 200.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.299
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
36 more

10 mentions without citation

Hox specificity unique roles for cofactors and collaborators.
Mann et al. (2009)
9 more