› 2r68

X-ray diffraction
2.4Å resolution

Complex Structure of Sucrose Phosphate Synthase (SPS)-S6P of Halothermothrix orenii

Released:
DOI: 10.2210/pdb2r68/pdb
PDB entry 2r68 coloured by chain, front view.
PDB entry 2r68 coloured by chain, side view.
PDB entry 2r68 coloured by chain, top view.
Source organism: Halothermothrix orenii H 168
Primary publication:
The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode.
Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J
Plant Cell 20 1059-72 (2008)
PMID: 18424616

Function and Biology Details

Reaction catalysed: 
UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
sucrose-phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 56.88 KDa
Source organism: Halothermothrix orenii H 168
Expression system: Escherichia coli
UniProt:
  • Canonical: B8CZ51 (Residues: 1-496; Coverage: 100%)
Gene name: Hore_18210
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, F6P
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 155.399Å b: 48.189Å c: 75.008Å
α: 90° β: 101.21° γ: 90°
R-values:
R R work R free
0.209 0.209 0.268
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Carbohydrate synthesis by disaccharide phosphorylases: reactions, catalytic mechanisms and application in the glycosciences.
Luley-Goedl et al. (2010)
4 more