› 2r6e

X-ray diffraction
5.02Å resolution

Crystal Form B2

Released:
DOI: 10.2210/pdb2r6e/pdb
PDB entry 2r6e coloured by chain, front view.
PDB entry 2r6e coloured by chain, side view.
PDB entry 2r6e coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase.
Bailey S, Eliason WK, Steitz TA
Science 318 459-63 (2007)
PMID: 17947583

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194911 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicative DNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 454 amino acids
Theoretical weight: 50.7 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X4C9 (Residues: 1-454; Coverage: 100%)
Gene name: dnaB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 199.797Å b: 199.797Å c: 195.036Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.394 0.393 0.397
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

SSB as an organizer/mobilizer of genome maintenance complexes.
Shereda et al. (2008)
37 more

2 mentions without citation

Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.
Huang et al. (2014)
1 more