› 2r6h

X-ray diffraction
2.95Å resolution

Crystal structure of the domain comprising the NAD binding and the FAD binding regions of the NADH:ubiquinone oxidoreductase, Na translocating, F subunit from Porphyromonas gingivalis

Released:
DOI: 10.2210/pdb2r6h/pdb
PDB entry 2r6h coloured by chain, front view.
PDB entry 2r6h coloured by chain, side view.
PDB entry 2r6h coloured by chain, top view.
Source organism: Porphyromonas gingivalis W83
Entry authors: Kim Y, Mulligan R, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
NADH + ubiquinone + n Na(+)(Side 1) = NAD(+) + ubiquinol + n Na(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)-translocating NADH-quinone reductase subunit F Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 290 amino acids
Theoretical weight: 33.82 KDa
Source organism: Porphyromonas gingivalis W83
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7MT22 (Residues: 126-412; Coverage: 70%)
Gene names: PG_2177, nqrF
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 11 x SO4
Ligand FAD 4 x FAD
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I4122
Unit cell:
a: 175.681Å b: 175.681Å c: 244.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.215 0.271
Expression system: Escherichia coli

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