› 2r6z

X-ray diffraction
1.8Å resolution

Crystal structure of the SAM-dependent methyltransferase NGO1261 from Neisseria gonorrhoeae, Northeast Structural Genomics Consortium Target NgR48

Released:
DOI: 10.2210/pdb2r6z/pdb
PDB entry 2r6z coloured by chain, front view.
PDB entry 2r6z coloured by chain, side view.
PDB entry 2r6z coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Entry authors: Forouhar F, Abashidze M, Seetharaman J, Mao L, Nwosu C, Fang Y, Xiao R, Baran MC, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase J Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 28.61 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: P72077 (Residues: 1-250; Coverage: 100%)
Gene name: rsmJ
Sequence domains: Putative SAM-dependent methyltransferase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 58.233Å b: 43.186Å c: 103.57Å
α: 90° β: 97.54° γ: 90°
R-values:
R R work R free
0.216 0.215 0.231
Expression system: Escherichia coli

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