› 2r78

X-ray diffraction
1.6Å resolution

Crystal structure of a domain of the sensory box sensor histidine kinase/response regulator from Geobacter sulfurreducens

Released:
DOI: 10.2210/pdb2r78/pdb
PDB entry 2r78 coloured by chain, front view.
PDB entry 2r78 coloured by chain, side view.
PDB entry 2r78 coloured by chain, top view.
Source organism: Geobacter sulfurreducens PCA
Entry authors: Zhang R, Sather A, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181052 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 117 amino acids
Theoretical weight: 12.88 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q74DN1 (Residues: 41-145; Coverage: 14%)
Gene name: GSU1285
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand ACT 10 x ACT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 51.248Å b: 62.501Å c: 71.203Å
α: 90° β: 94.82° γ: 90°
R-values:
R R work R free
0.181 0.179 0.221
Expression system: Escherichia coli BL21

Quick links