› 2r7d

X-ray diffraction
1.8Å resolution

Crystal structure of ribonuclease II family protein from Deinococcus radiodurans, triclinic crystal form. NorthEast Structural Genomics target DrR63

Released:
DOI: 10.2210/pdb2r7d/pdb
PDB entry 2r7d coloured by chain, front view.
PDB entry 2r7d coloured by chain, side view.
PDB entry 2r7d coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
The structure and enzymatic properties of a novel RNase II family enzyme from Deinococcus radiodurans.
Schmier BJ, Seetharaman J, Deutscher MP, Hunt JF, Malhotra A
J Mol Biol 415 547-59 (2012)
PMID: 22133431

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease II/R domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 469 amino acids
Theoretical weight: 52.2 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RYD0 (Residues: 1-461; Coverage: 100%)
Gene name: DR_0020
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 65.762Å b: 92.205Å c: 92.345Å
α: 60.08° β: 89.57° γ: 71.11°
R-values:
R R work R free
0.221 0.221 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Intracellular ribonucleases involved in transcript processing and decay: precision tools for RNA.
Arraiano et al. (2013)
2 other articles

1 mention without citation

The structure and enzymatic properties of a novel RNase II family enzyme from Deinococcus radiodurans.
Schmier et al. (2012)