› 2ra5

X-ray diffraction
2.4Å resolution

Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb2ra5/pdb
PDB entry 2ra5 coloured by chain, front view.
PDB entry 2ra5 coloured by chain, side view.
PDB entry 2ra5 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Entry authors: Kim Y, Xu X, Zheng H, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH gntR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.01 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RKT6 (Residues: 1-245; Coverage: 100%)
Gene names: SCAH10.21, SCO6256
Sequence domains:
Structure domains: Chorismate lyase-like

Ligands and Environments

2 bound ligands:
Ligand SRT 1 x SRT
Ligand IPA 1 x IPA
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 43.768Å b: 43.768Å c: 170.735Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.218 0.292
Expression system: Escherichia coli BL21(DE3)

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