› 2rbi

X-ray diffraction
2.2Å resolution

STRUCTURE OF BINASE MUTANT HIS 101 ASN

Released:
DOI: 10.2210/pdb2rbi/pdb
PDB entry 2rbi coloured by chain, front view.
PDB entry 2rbi coloured by chain, side view.
PDB entry 2rbi coloured by chain, top view.
Source organism: Bacillus intermedius
Primary publication:
RNA cleavage without hydrolysis. Splitting the catalytic activities of binase with Asn101 and Thr101 mutations.
Okorokov AL, Panov KI, Offen WA, Mukhortov VG, Antson AA, Wilkinson AJ, Dodson GG
Protein Eng 10 273-8 (1997)
PMID: 9153077

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 12.2 KDa
Source organism: Bacillus intermedius
Expression system: Escherichia coli
UniProt:
  • Canonical: P00649 (Residues: 54-162; Coverage: 82%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 111.26Å b: 69.15Å c: 33.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Enzyme genomics: Application of general enzymatic screens to discover new enzymes.
Kuznetsova et al. (2005)
8 other articles

1 mention without citation

Sialidase-like Asp-boxes: sequence-similar structures within different protein folds.
Copley et al. (2001)