› 2rcc

X-ray diffraction
1.9Å resolution

Crystal structure of putative class I ribonucleotide reductase (NP_241368.1) from Bacillus halodurans at 1.90 A resolution

Released:
DOI: 10.2210/pdb2rcc/pdb
PDB entry 2rcc coloured by chain, front view.
PDB entry 2rcc coloured by chain, side view.
PDB entry 2rcc coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-191860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit beta Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 346 amino acids
Theoretical weight: 40.86 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KFH7 (Residues: 1-345; Coverage: 100%)
Gene names: BH0502, nrdB
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

6 bound ligands:
Ligand PG4 1 x PG4
Ligand PEG 6 x PEG
Ligand ZN 4 x ZN
Ligand EDO 1 x EDO
Ligand PGE 1 x PGE
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 33 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 175.26Å b: 72.78Å c: 111.77Å
α: 90° β: 124.9° γ: 90°
R-values:
R R work R free
0.184 0.183 0.215
Expression system: Escherichia coli

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