› 2rcf

X-ray diffraction
2.15Å resolution

Carboxysome Shell protein, OrfA from H. Neapolitanus

Released:
DOI: 10.2210/pdb2rcf/pdb
PDB entry 2rcf coloured by chain, front view.
PDB entry 2rcf coloured by chain, side view.
PDB entry 2rcf coloured by chain, top view.
Source organism: Halothiobacillus neapolitanus
Primary publication:
Atomic-level models of the bacterial carboxysome shell.
Tanaka S, Kerfeld CA, Sawaya MR, Cai F, Heinhorst S, Cannon GC, Yeates TO
Science 319 1083-6 (2008)
PMID: 18292340

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxysome shell vertex protein CsoS4A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 91 amino acids
Theoretical weight: 9.97 KDa
Source organism: Halothiobacillus neapolitanus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O85043 (Residues: 1-83; Coverage: 100%)
Gene names: Hneap_0918, csoS4A, orfA
Sequence domains: Ethanolamine utilisation protein EutN/carboxysome
Structure domains: EutN/Ccml

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 61.933Å b: 86.78Å c: 35.214Å
α: 90° β: 90.22° γ: 90°
R-values:
R R work R free
0.187 0.184 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

52 review citations

Ecological genomics of marine picocyanobacteria.
Scanlan et al. (2009)
51 more

4 mentions without citation

Comparative analysis of carboxysome shell proteins.
Kinney et al. (2011)
3 more