› 2rcy

X-ray diffraction
2.3Å resolution

Crystal structure of Plasmodium falciparum pyrroline carboxylate reductase (MAL13P1.284) with NADP bound

Released:
DOI: 10.2210/pdb2rcy/pdb
PDB entry 2rcy coloured by chain, front view.
PDB entry 2rcy coloured by chain, side view.
PDB entry 2rcy coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Entry authors: Wernimont AK, Lew J, Lin YH, Ren H, Sun X, Khuu C, Hassanali A, Wasney G, Zhao Y, Kozieradzki I, Schapira M, Bochkarev A, Weigelt J, Sundstrom M, Arrowsmith CH, Edwards AM, Hui R, Artz JD, Amani M, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrroline-5-carboxylate reductase, putative Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 262 amino acids
Theoretical weight: 28.81 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IDC6 (Residues: 1-261; Coverage: 100%)
Gene name: PF3D7_1357900
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 5 x NAP
2 bound ligands:
Ligand MG 4 x MG
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 50 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 160.207Å b: 113.741Å c: 82.651Å
α: 90° β: 105.71° γ: 90°
R-values:
R R work R free
0.247 0.245 0.289
Expression system: Escherichia coli

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