› 2rdd

X-ray diffraction
3.5Å resolution

X-ray crystal structure of AcrB in complex with a novel transmembrane helix.

Released:
DOI: 10.2210/pdb2rdd/pdb
PDB entry 2rdd coloured by chain, front view.
PDB entry 2rdd coloured by chain, side view.
PDB entry 2rdd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist.
Törnroth-Horsefield S, Gourdon P, Horsefield R, Brive L, Yamamoto N, Mori H, Snijder A, Neutze R
Structure 15 1663-73 (2007)
PMID: 18073115

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:
Sec translocon accessory complex subunit YajC Chain: B
Molecule details ›
Chain: B
Length: 37 amino acids
Theoretical weight: 4.45 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ7 (Residues: 19-55; Coverage: 34%)
Gene names: JW0397, b0407, yajC
Sequence domains: Preprotein translocase subunit

Ligands and Environments

1 bound ligand:
Ligand AIC 2 x AIC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: R32
Unit cell:
a: 145.097Å b: 145.097Å c: 511.644Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.279 0.279 0.317

Quick links

Citations

23 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
22 more

6 mentions without citation

The Sec System: Protein Export in Escherichia coli.
Crane et al. (2017)
5 more