› 2rde

X-ray diffraction
1.92Å resolution

Crystal structure of VCA0042 complexed with c-di-GMP

Released:
DOI: 10.2210/pdb2rde/pdb
PDB entry 2rde coloured by chain, front view.
PDB entry 2rde coloured by chain, side view.
PDB entry 2rde coloured by chain, top view.
Source organism: Vibrio cholerae O395
Primary publication:
The structural basis of cyclic diguanylate signal transduction by PilZ domains.
Benach J, Swaminathan SS, Tamayo R, Handelman SK, Folta-Stogniew E, Ramos JE, Forouhar F, Neely H, Seetharaman J, Camilli A, Hunt JF
EMBO J 26 5153-66 (2007)
PMID: 18034161

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic di-GMP binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 28.25 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3ADR8 (Residues: 23-252; Coverage: 91%)
Gene name: VC0395_0091
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand C2E 2 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 47.006Å b: 55.4Å c: 58.044Å
α: 99.88° β: 97.09° γ: 106.8°
R-values:
R R work R free
0.195 0.195 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
27 more

25 mentions without citation

Diversity of Cyclic Di-GMP-Binding Proteins and Mechanisms.
Chou et al. (2016)
24 more