› 2rf5

X-ray diffraction
2.3Å resolution

Crystal structure of human tankyrase 1- catalytic PARP domain

Released:
DOI: 10.2210/pdb2rf5/pdb
PDB entry 2rf5 coloured by chain, front view.
PDB entry 2rf5 coloured by chain, side view.
PDB entry 2rf5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Zinc binding catalytic domain of human tankyrase 1.
Lehtiö L, Collins R, van den Berg S, Johansson A, Dahlgren LG, Hammarström M, Helleday T, Holmberg-Schiavone L, Karlberg T, Weigelt J
J Mol Biol 379 136-45 (2008)
PMID: 18436240

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase tankyrase-1 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95271 (Residues: 1091-1325; Coverage: 18%)
Gene names: PARP5A, PARPL, TIN1, TINF1, TNKS, TNKS1
Sequence domains: Poly(ADP-ribose) polymerase catalytic domain
Structure domains: Phosphoenolpyruvate Carboxykinase; domain 3

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I212121
Unit cell:
a: 79.95Å b: 81.24Å c: 82.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.25
Expression system: Escherichia coli

Quick links

Citations

6 review citations

PARP inhibition: PARP1 and beyond.
Rouleau et al. (2010)
5 more

16 mentions without citation

The PARP side of the nucleus: molecular actions, physiological outcomes, and clinical targets.
Krishnakumar et al. (2010)
15 more