› 2rfl

X-ray diffraction
2.35Å resolution

Crystal structure of the putative phosphohistidine phosphatase SixA from Agrobacterium tumefaciens

Released:
DOI: 10.2210/pdb2rfl/pdb
PDB entry 2rfl coloured by chain, front view.
PDB entry 2rfl coloured by chain, side view.
PDB entry 2rfl coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Entry authors: Kim Y, Binkowski T, Xu X, Edwards AM, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphohistidine phosphatase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 173 amino acids
Theoretical weight: 19.18 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9CJ67 (Residues: 1-169; Coverage: 100%)
Gene name: Atu1358
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

3 bound ligands:
Ligand SCN 1 x SCN
Ligand GOL 2 x GOL
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 58.966Å b: 201.503Å c: 72.717Å
α: 90° β: 113.98° γ: 90°
R-values:
R R work R free
0.236 0.23 0.283
Expression system: Escherichia coli BL21(DE3)

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