› 2rg7

X-ray diffraction
2.05Å resolution

Apo- Crystal Structure of a Periplasmic Heme Binding Protein from Shigella dysenteriae

Released:
DOI: 10.2210/pdb2rg7/pdb
PDB entry 2rg7 coloured by chain, front view.
PDB entry 2rg7 coloured by chain, side view.
PDB entry 2rg7 coloured by chain, top view.
Source organism: Shigella dysenteriae
Primary publication:
Holo- and apo-bound structures of bacterial periplasmic heme-binding proteins.
Ho WW, Li H, Eakanunkul S, Tong Y, Wilks A, Guo M, Poulos TL
J Biol Chem 282 35796-802 (2007)
PMID: 17925389

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 256 amino acids
Theoretical weight: 27.58 KDa
Source organism: Shigella dysenteriae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O70018 (Residues: 49-304; Coverage: 84%)
Gene names: NCTC4837_05544, shuT
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P1
Unit cell:
a: 54.817Å b: 73.28Å c: 72.38Å
α: 71.44° β: 77.8° γ: 89.9°
R-values:
R R work R free
0.232 0.232 0.274
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Iron uptake and metabolism in pseudomonads.
Cornelis P. (2010)
10 more

3 mentions without citation

Characterization of a Bacillus subtilis transporter for petrobactin, an anthrax stealth siderophore.
Zawadzka et al. (2009)
2 more