› 2rgj

X-ray diffraction
2.4Å resolution

Crystal structure of flavin-containing monooxygenase PhzS

Released:
DOI: 10.2210/pdb2rgj/pdb
PDB entry 2rgj coloured by chain, front view.
PDB entry 2rgj coloured by chain, side view.
PDB entry 2rgj coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of the pyocyanin biosynthetic protein PhzS.
Greenhagen BT, Shi K, Robinson H, Gamage S, Bera AK, Ladner JE, Parsons JF
Biochemistry 47 5281-9 (2008)
PMID: 18416536

Function and Biology Details

Reaction catalysed: 
5-methylphenazine-1-carboxylate + NADH + O(2) = pyocyanine + NAD(+) + CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methylphenazine-1-carboxylate 1-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 402 amino acids
Theoretical weight: 44.07 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HWG9 (Residues: 1-402; Coverage: 100%)
Gene names: PA4217, phzS
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I222
Unit cell:
a: 63.5Å b: 64.86Å c: 182.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.262
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Metabolism and function of phenazines in bacteria: impacts on the behavior of bacteria in the environment and biotechnological processes.
Pierson et al. (2010)
10 more

10 mentions without citation

Form follows function: structural and catalytic variation in the class a flavoprotein monooxygenases.
Crozier-Reabe et al. (2012)
9 more