› 2rgk

X-ray diffraction
2.5Å resolution

Functional annotation of Escherichia coli yihS-encoded protein

Released:
DOI: 10.2210/pdb2rgk/pdb
PDB entry 2rgk coloured by chain, front view.
PDB entry 2rgk coloured by chain, side view.
PDB entry 2rgk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of YihS in complex with D-mannose: structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase.
Itoh T, Mikami B, Hashimoto W, Murata K
J Mol Biol 377 1443-59 (2008)
PMID: 18328504

Function and Biology Details

Reaction catalysed: 
Beta-sulfoquinovose = 6-deoxy-6-sulfo-D-fructose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfoquinovose isomerase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 421 amino acids
Theoretical weight: 48.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32140 (Residues: 1-413; Coverage: 100%)
Gene names: JW5569, b3880, yihS
Sequence domains: N-acylglucosamine 2-epimerase (GlcNAc 2-epimerase)
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:
Ligand EPE 6 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 245.87Å b: 142.158Å c: 94.591Å
α: 90° β: 107.61° γ: 90°
R-values:
R R work R free
0.18 0.18 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Isomerases for biotransformation of D-hexoses.
Mu et al. (2015)
1 more

2 mentions without citation

Molecular Basis of Sulfosugar Selectivity in Sulfoglycolysis.
Sharma et al. (2021)
1 more